Electroacupuncture postpones growth of tumors by activating SIRT1 expression to reduce inflammatory reaction and facilitate cellular autophagy in mice with transformed colorectal carcinomas

• Published in: Zhēncì yánjiū Vol. 46; no. 12; p. 996
• Main Authors: Li, Jin-Xiao, Liu, Na, Zhou, Min-Feng, Li, Hua-Rong, Zhou, Cong, Zhang, Hai-Ming, Huang, Qi, Yan, Jiang-Tian, Han, Ying, Liang, Feng-Xia, Chen, Rui
• Format: Journal Article
• Language: Chinese
• Published: China 25.12.2021
• Subjects: Acupuncture Points; Animals; Autophagy - genetics; Colorectal Neoplasms - genetics; Colorectal Neoplasms - therapy; Electroacupuncture; Inflammation - genetics; Inflammation - therapy; Male; Mice; Mice, Inbred C57BL; Sirtuin 1 - genetics; Silent information regulator 1 (SIRT1); Electroacupuncture; Autophagy; Colorectal cancer; Colorectal inflammatory factors
• ISSN: 1000-0607
• DOI: 10.13702/j.1000-0607.20210633

To observe the effect of electroacupuncture (EA) on tumor number, body conditions, inflammatory factors and expression levels of silent information regulator 1 (sirtuin 1, SIRT1) and autophagy-related proteins Beclin-1, P62, and LC3 in colorectal tissues in inflammatory-transformed colorectal cancer mice, so as to explore its underlying mechanisms in resisting tumor growth. A total of 100 C57BL/6 male mice were randomly divided into normal control, model, EA, EA + SIRT1 inhibitor (EA+inhibitor) and agonist resveratrol (agonist) groups, with 20 mice in each group. EA (2 Hz, 1 mA) was applied to "Zusanli"(ST36)and "Fenglong"(ST40) for 20 min every time, 3 times a week for 11 weeks. Mice of the EA +inhibitor group received intraperitoneal injection of SIRT1 inhibitor EX527 (5 mg/kg) at the same time of EA treatment, and those of the agonist group received gavage of resveratrol (200 mg/kg, an agonist of SIRT1), 3 times a week for 11 weeks. The body mass was measured weekly. The disease activity index (DAI), color