Effects of Notch-1/Twist-1 axis in the process of epithelial-mesenchymal transition of type II alveolar epithelial cell and its mechanism

• Published in: Zhongguo ying yong sheng li xue za zhi Vol. 37; no. 6; p. 577
• Main Authors: Tang, Juan, Ke, Jie, Fu, Yu, He, Xiao-Wei, Li, Xian-Wei
• Format: Journal Article
• Language: Chinese
• Published: China 01.11.2021
• Subjects: Alveolar Epithelial Cells - cytology; Animals; Bleomycin; Epithelial-Mesenchymal Transition; Lung; Pulmonary Fibrosis - chemically induced; Rats; Receptor, Notch1 - metabolism; Signal Transduction; Transforming Growth Factor beta1; Twist-Related Protein 1 - metabolism; Twist-1; epithelial-mesenchymal transition; rat; Notch-1; pulmonary fibrosis
• ISSN: 1000-6834
• DOI: 10.12047/j.cjap.6124.2021.048

To study the role of Notch-1/Twist-1 axis in the process of epithelial-mesenchymal transition (EMT) of type II alveolar epithelial cells in pulmonary fibrosis (PF) and hope to provide a new theoretical basis for the pathogenesis of PF. Thirty rats were randomly divided into control group and bleomycin (BLM) group, 15 rats in each group. The PF rat model was induced by intratracheal injection of BLM (7 500 U/kg). Excised inferior lobe of left lung was fixed in 10% formalin for HE staining, Masson staining and transforming growth factor-beta 1 (TGF-β1) immunohistochemistry staining after BLM injection for 28 days. The cultured type II alveolar epithelial cells (RLE-6TN) were divided into 4 groups (Control group, transforming growth factor-beta 1 (TGF-β1) group, Notch-1 negative control siRNA (NC siRNA, 100 pmol/L) group and Notch-1 siRNA (100 pmol/L) group), each group was established nine holes. The cells were treated with TGF-β1 (5.0 ng/ml) for 24 h following NC siRNA or Notch-1 siRNA for 48 h. The mRNA and (