Role of Pim1 Gene Overexpression in Pathogenesis of Acute Myeloid Leukemia
To investigate the effect of Pim1 expression up-regulation on cell proliferation, apoptosis, chemotaxis and angiogenesis in acute myeloid leukemia (AML) cell line U937, and to explore the possible molecular mechanism involved, finally to estimate the Pim1 expression in primary AML cells. GFP-tagged...
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Published in | Zhongguo shi yan xue ye xue za zhi Vol. 27; no. 3; p. 664 |
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Main Authors | , , , , |
Format | Journal Article |
Language | Chinese |
Published |
China
01.06.2019
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Subjects | |
Online Access | Get more information |
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Summary: | To investigate the effect of Pim1 expression up-regulation on cell proliferation, apoptosis, chemotaxis and angiogenesis in acute myeloid leukemia (AML) cell line U937, and to explore the possible molecular mechanism involved, finally to estimate the Pim1 expression in primary AML cells.
GFP-tagged plasmid for Pim1 overexpression and an empty vector plasmid were constructed, and then a stable Pim1 expressed U937 cell line and a control virus-infected U937 cell line were established by a lentiviral vector system. After confirming Pim1 overexpression in U937 cells, proliferation and apoptosis are determined by CCK-8 Kit and flow cytometry respectively. Transwell chemotaxis assay was used to measure the effect of Pim1 overexpression on AML cells. Flow cytometry and confocal microscopy were applied to detect the influence of Pim1 overexpression on phosphorylated CXCR4 (pCXCR4) and its location. Real-time fluorescence quantitative PCR (qPCR) was used to detect the expression of angiogenesis and adhesion related ge |
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ISSN: | 1009-2137 |
DOI: | 10.19746/j.cnki.issn.1009-2137.2019.03.006 |