Mechanism of antitumor effect of ursolic acid on T (8;21) leukemia cell kasumi-1
This study was purposed to investigate the anti-tumor effect of ursolic acid (UA) on t(8;21) leukemia cell line kasumi-1 and its possible mechanisms. The kasumi-1 cells were treated with UA at different concentration for different duration of time. The growth inhibition of kasumi-1 treated with UA w...
Saved in:
Published in | Zhongguo shi yan xue ye xue za zhi Vol. 22; no. 3; p. 687 |
---|---|
Main Authors | , , , , , |
Format | Journal Article |
Language | Chinese |
Published |
China
01.06.2014
|
Subjects | |
Online Access | Get more information |
Cover
Loading…
Summary: | This study was purposed to investigate the anti-tumor effect of ursolic acid (UA) on t(8;21) leukemia cell line kasumi-1 and its possible mechanisms. The kasumi-1 cells were treated with UA at different concentration for different duration of time. The growth inhibition of kasumi-1 treated with UA was detected by using CCK-8 test, and the morphological changes of kasumi-1 cells were observed by Wright's staining. Furthermore, the apoptosis rate of kasumi-1 was examined by flow cytometry. Lastly, the expression of AML1-ETO, KIT, MYC, CCND1, BCL-2, P53, BAX, MDM2 and protein were detected by using real-time quantitative PCR and Western blot respectively. The results showed that the UA obviously inhibited the growth of kasumi-1 cells in dose- and time-dependent manners. The apoptotic morphological changes of cells were presented when kasumi-1 cells were treated with UA for 48 hours. The apoptotic rate of kasumi-1 cells increased in a dose- and time-dependent ways, and the mRNA levels of AML1-ETO, KIT, MYC, CCND1 |
---|---|
ISSN: | 1009-2137 |
DOI: | 10.7534/j.issn.1009-2137.2014.03.021 |