Mechanism of antitumor effect of ursolic acid on T (8;21) leukemia cell kasumi-1

• Published in: Zhongguo shi yan xue ye xue za zhi Vol. 22; no. 3; p. 687
• Main Authors: Zhang, Jun-Feng, Gao, Li, Duan, Hao-Qing, Wang, Wei, Li, Yan, Ma, Yi-Gai
• Format: Journal Article
• Language: Chinese
• Published: China 01.06.2014
• Subjects: Apoptosis - drug effects; Cell Line, Tumor; Cell Proliferation - drug effects; Humans; Leukemia - genetics; Oncogene Proteins, Fusion - genetics; Translocation, Genetic; Triterpenes - pharmacology; Ursolic Acid
• ISSN: 1009-2137
• DOI: 10.7534/j.issn.1009-2137.2014.03.021

This study was purposed to investigate the anti-tumor effect of ursolic acid (UA) on t(8;21) leukemia cell line kasumi-1 and its possible mechanisms. The kasumi-1 cells were treated with UA at different concentration for different duration of time. The growth inhibition of kasumi-1 treated with UA was detected by using CCK-8 test, and the morphological changes of kasumi-1 cells were observed by Wright's staining. Furthermore, the apoptosis rate of kasumi-1 was examined by flow cytometry. Lastly, the expression of AML1-ETO, KIT, MYC, CCND1, BCL-2, P53, BAX, MDM2 and protein were detected by using real-time quantitative PCR and Western blot respectively. The results showed that the UA obviously inhibited the growth of kasumi-1 cells in dose- and time-dependent manners. The apoptotic morphological changes of cells were presented when kasumi-1 cells were treated with UA for 48 hours. The apoptotic rate of kasumi-1 cells increased in a dose- and time-dependent ways, and the mRNA levels of AML1-ETO, KIT, MYC, CCND1