Toxoplasma gondii Infection in HIV-infected Individuals in Henan Province

• Published in: Zhongguo jishengchongxue yu jishengchongping zazhi Vol. 34; no. 1; p. 84
• Main Authors: Bai, Chen-qian, Wang, Dong, Yao, Zhi-jun, Wang, Shuai, Zhang, Lu-wen, Diao, Feng-jin, Ren, Xiao-ying, He, Chan-chan, Wang, Zhao-hong, Ma, Jin-chao, Wang, Su-kang, Peng, Bang-gan, Hu, Cui, An, Ge, Liu, Shi-guo
• Format: Journal Article
• Language: Chinese
• Published: China 01.02.2016
• ISSN: 1000-7423

Genomic DNA was extracted from 1 038 peripheral blood samples from HIV-infected individuals in Henan Province. One-step single-tube nested PCR was performed to amplify the 529 bp repeating sequences of Toxoplasma gondii. Of the 1 038 samples （762 from males and 276 from females）, 66 showed positive PCR results, with a positive rate of 6.4%. The PCR positive rate in males and females was 6.3% （48/762） and 6.5% （18/276） respectively. The PCR positive rate in the married HIV individuals was 4.9%（25/508）, and that in unmarried, divorced and widowed HIV individuals was 7.7% （41/530）（χ2 = 3.451, P> 0.05）. The PCR positive rate in HIV individuals with a high-school educational level or above was 6.9%（34/489）, and that in those below the high-school level was 5.8% （32/549）（χ2 = 0.545, P> 0.05）. The highest infection rate was in the age group of 20-40 years（7.6%, 31/410）. In addition, the Toxoplasma infection rate in those with and without a history of venereal diseases, and those with an unknown history was 8.0%（9/11