Effects of mTOR Inhibitor Rapamycin on Burkitt's Lymphoma Cells

To explore the effects of mTOR inhibitor rapamycin on proliferation, cell cycle and apoptosis of Burkitt's lymphoma cell line Raji and CA46 cells and its mechanism, so as to provide the experimental evidence for a therapeutic target of Burkitt's lymphoma. 3-(4,5-dimethylthiazol-2-yl)-2, 5-...

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Published inZhongguo shi yan xue ye xue za zhi Vol. 25; no. 5; p. 1397
Main Authors Zhou, Lun-Huan, Zhu, Xiong-Peng, Xiao, Hui-Fang, Xin, Peng-Liang, Li, Chun-Tuan
Format Journal Article
LanguageChinese
Published China 01.10.2017
Subjects
Antibiotics, Antineoplastic - pharmacology
Apoptosis - drug effects
Burkitt Lymphoma - drug therapy
Cell Line, Tumor
Cell Proliferation
Humans
Sirolimus - pharmacology
TOR Serine-Threonine Kinases
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Summary:To explore the effects of mTOR inhibitor rapamycin on proliferation, cell cycle and apoptosis of Burkitt's lymphoma cell line Raji and CA46 cells and its mechanism, so as to provide the experimental evidence for a therapeutic target of Burkitt's lymphoma. 3-(4,5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide(MTT) assay was performed to assess the inhibitory effect of rapamycin on proliferation of Burkitt's lymphoma cell line Raji and CA46 cells. The cell cycle distribution of Raji and CA46 cells was analyzed by flow cytometry with propidium iodide(PI) single staining. The cell apoptosis of Raji and CA46 cells was analyzed by flow cytometry with FITC Annexin V+PI double staining. The expressions of RPS6, p-RPS6, survivin and caspase-3 proteins were detected by Western blot after treating with rapamycin. Rapamycin markedly inhibited the proliferation of both Raji and CA46 cells in a time- and concentration-dependent manners, showing good biological activity, the cell proliferation inhibition rate reache
ISSN:1009-2137
DOI:10.7534/j.issn.1009-2137.2017.05.020