Protective effects of ulinastatin against acute lung injury induced by heatstroke in mice

• Published in: Nan fang yi ke da xue xue bao = Journal of Southern Medical University Vol. 35; no. 9; p. 1277
• Main Authors: Zhou, Gengbiao, Xu, Qiulin, Liu, Yanan, Wang, Zhenglian, Su, Lei, Guo, Xiaohua
• Format: Journal Article
• Language: Chinese
• Published: China 01.08.2015
• Subjects: Acute Lung Injury - drug therapy; Acute Lung Injury - physiopathology; Animals; Body Temperature; Bronchoalveolar Lavage Fluid - chemistry; Edema - prevention & control; Glycoproteins - therapeutic use; Heat Stroke - physiopathology; Lung - pathology; Mice; Mice, Inbred C57BL
• ISSN: 1673-4254

To investigate the protective effect of ulinastatin (UTI) against acute lung injury induced by heatstroke in mice. Sixty C57/BL6 mice were randomly divided into 6 groups, with 10 mice in each: control group, heatstroke group, UTI pretreatment group, saline pretreatment group, UTI post-treatment group, saline post-treatment group. The control mice were housed at a controlled room temperature of (22∓1) degrees; celsius, and the other groups were placed inside a temperature and humidity controlled chamber pre-set at 37 degrees; celsius and 60%. The two UTI groups were intraperitoneally injected with UTI at 5×10(4) U/kg 10 min before or after heat stress, and the two saline groups were given then equal amounts of saline in the same manner. The core body temperature of mice was monitored by a mercury thermometer every 30 min in the first 1.5 h during heating. The core temperature was measured, then every 15 min until it reached 42.7 degrees; celsius, which was taken as the onset of heatstroke. The animals were all