Expression of ANO1 during cardiac fibroblasts differentiation and its electrophysiological characteristics as calcium-activated chloride channel protein

• Published in: Zhongguo ying yong sheng li xue za zhi Vol. 36; no. 4; p. 358
• Main Authors: Tian, Xiang-Qin, Tan, Chao-Yang, Li, Na-Na, Chang, Yu-Qiao, Zhang, Ai-Mei, Zhou, Ying, Ma, Ke-Tao, Si, Jun-Qiang
• Format: Journal Article
• Language: Chinese
• Published: China 01.07.2020
• Subjects: Animals; Anoctamin-1 - metabolism; Calcium - metabolism; Cell Differentiation; Chloride Channels; Fibroblasts - metabolism; Rats; calcium-activated chloride channels; ANO1; cardiac fibroblasts; whole-cell patch clamp; myocardial fibrosis
• ISSN: 1000-6834
• DOI: 10.12047/j.cjap.5940.2020.077

To investigate the expression and electrophysiological characteristics of calcium-activated chlorine channel anoctamin-1 (ANO1) protein during the differentiation of cardiac fibroblasts (CFs) into myofibroblasts (MFs), and to elucidate the role of ANO1 in myocardial fibrosis. The primary CFs from neonatal rats were isolated and the cells differentiated into MFs by subculture. The Ca -activated Cl current ( ) in CFs and MFs were measured by whole-cell patch clamp, and the expressions of ANO1, α-smooth muscle actin(α-SMA)and vimentin in CFs and MFs were detected by immunofluorescence assay and Western blot, respectively. The current density in the early adherent CFs was stronger than that in MFs. ANO1 was expressed preferentially within and around the nuclei, and a small amount of ANO1 was expressed on the cell membrane. Moreover, ANO1 expression was weak in the early adherent CFs and displayed stronger expression in the MFs with proliferation tendency. The expression of ANO1 is closely related to the different