Kinetic distribution of cyst-forming Chinese isolate of Toxoplasma gondii in mice

To determine the kinetics of infection and cyst formation in CD1 mice following oral infection with cyst-forming Chinese isolate of Toxoplasma gondii TgCtwh1(genotype China 1, ToxoDB#9). 50 CD1 female mice were obtained from specific pathogen-free (SPF) mouse colony in the Vital River Laboratories (...

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Published inZhongguo jishengchongxue yu jishengchongping zazhi Vol. 30; no. 3; p. 179
Main Authors Wang, Lin, Huo, Xing-Xing, Chen, Zhao-Wu, Chen, He, Yu, Li, Song, Xiao-Rong, Faustina, Halm-Lai, Luo, Qing-Li, Shen, Ji-Long
Format Journal Article
LanguageChinese
Published China 30.06.2012
Subjects
Animals
Brain - parasitology
Female
Genes, Protozoan
Genotype
Mice
Polymerase Chain Reaction - methods
Toxoplasma - genetics
Toxoplasma - isolation & purification
Toxoplasmosis, Animal - parasitology
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Summary:To determine the kinetics of infection and cyst formation in CD1 mice following oral infection with cyst-forming Chinese isolate of Toxoplasma gondii TgCtwh1(genotype China 1, ToxoDB#9). 50 CD1 female mice were obtained from specific pathogen-free (SPF) mouse colony in the Vital River Laboratories (VRL), Beijing. Mice were randomly divided into 10 groups each with 5 mice. All mice but control were peroral gavage infected with 50 cysts (1x10(4) bradyzoites) of TgCtwh1 isolate of T. gondii isolated from Wuhan, China. Cysts were isolated from the entire brain of mice infected with TgCtwh1 by density gradient centrifugation over Fycoll-paque plus. Animals were orally inoculated with cysts on day zero, and peripheral blood, lymph nodes, heart, liver, and brain of infected mice were collected on days 2, 4, 7, 10, 14, 21, 35, 50, and 72 post infection. Five mice were sacrificed by cervical dislocation under anesthesia at each time of collection, and the kinetic distribution was detected by fluorescence quantitative
ISSN:1000-7423