Microbial community in nitrogen cycle of aquaculture water of the Pearl River Delta

In order to study the characteristic of nitrogen transport, the community structure and diversity of related microorganisms in aquaculture water of the Pearl River Delta. We established an artificial aquaculture ecosystem to study the microbial community of 15N-stable isotope probing (15N-SIP) label...

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Bibliographic Details
Published inWei sheng wu hsüeh pao Vol. 52; no. 5; p. 645
Main Authors Cai, Xiaolong, Luo, Jianfei, Lin, Weitie, Tian, Guoliang
Format Journal Article
LanguageChinese
Published China 04.05.2012
Subjects
Aquaculture
Archaea - genetics
Archaea - isolation & purification
Archaea - metabolism
Bacteria - genetics
Bacteria - isolation & purification
Bacteria - metabolism
China
Ecosystem
Nitrogen - metabolism
Nitrogen Cycle
Phylogeny
Rivers - microbiology
China
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Summary:In order to study the characteristic of nitrogen transport, the community structure and diversity of related microorganisms in aquaculture water of the Pearl River Delta. We established an artificial aquaculture ecosystem to study the microbial community of 15N-stable isotope probing (15N-SIP) labeled nitrogen transport microorganisms. The 15N-labeled DNA was separated by CsCl-ethidium bromide density gradient centrifugation, and was used to construct 16S rRNA gene clone libraries of bacteria and archaea. Phylogenetic analysis shows that 19 Operational Taxonomic Units (OTUs) from bacterial library were clustered in Proteobacteria and Planctomycetes. Proteobacteria (99.2%) was the dominant group, mainly consisted of Comamonas (15.7%), Nitrosomonas (12.4%), Enterobacteriaceae (11.5%) and Nitrobacter (11.5%). From archaeal library 9 OTUs were divided into 3 phyla: Thaumarchaeota, Crenarchaeota and Euryarchaeota. We successfully elucidated the microbial community of nitrogen transport microorganisms in aquacultur
ISSN:0001-6209