D(1)-dopamine receptor is involved in the modulation of the respiratory rhythmical discharge activity in the medulla oblongata slice preparation of neonatal rats in vitro.

To explore the role of D(1)-dopamine receptor in the modulation of basic respiratory rhythm, neonatal (0-3 d) Sprague-Dawley rats of either sex were used. The medulla oblongata slice was prepared and the surgical procedure was performed in the modified Kreb's solution (MKS) with continuous vent...

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Bibliographic Details
Published inSheng li hsüeh pao Vol. 60; no. 6; p. 704
Main Authors Jiao, Yong-Gang, Wu, Zhong-Hai
Format Journal Article
LanguageChinese
Published China 25.12.2008
Subjects
Animals
Animals, Newborn
Benzazepines - pharmacology
Biological Clocks
Chromans - pharmacology
Female
In Vitro Techniques
Male
Medulla Oblongata - physiology
Rats
Rats, Sprague-Dawley
Receptors, Dopamine - physiology
Respiration
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Summary:To explore the role of D(1)-dopamine receptor in the modulation of basic respiratory rhythm, neonatal (0-3 d) Sprague-Dawley rats of either sex were used. The medulla oblongata slice was prepared and the surgical procedure was performed in the modified Kreb's solution (MKS) with continuous ventilating 95% O2 and 5% CO2 and ended in 3 min. A 600-700 mum single transverse slice containing the hypoglossal nerve roots and some parts of the ventral respiratory group was cut. The preparation was quickly transferred to a recording chamber and continuously perfused with oxygen-saturated MKS at a rate of 4-6 mL/min at 27-29 degrees C. Ten medulla oblongata slice preparations were randomly divided into two groups. In group I, the preparations were perfused with perfusion solution containing D(1)-dopamine receptor specific agonist cis-(+/-)-1-(Aminomethyl)-3,4-dihydro-3-phenyl-1H-2-Benzopyran-5,6-Diolhy-drochlo-ride (A68930, 5 mumol/L) for 10 min first; after washing out, the preparations were then perfused with perfusi
ISSN:0371-0874