Effect and mechanism of ghrelin and its synthetic peptide growth hormone releasing peptide 6 on gastric motor in mice

To investigate the effect and mechanism of ghrelin and its synthetic peptide GHRP-6 on gastric motor in mice. In vivo, the dose-dependent effects of ghrelin (20,50,100,200 mug/kg) and GHRP-6 (20,50,100,200 mug/kg) on gastric emptying were measured by intragastric application of phenol red test which...

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Bibliographic Details
Published inZhonghua wei chang wai ke za zhi Vol. 11; no. 2; p. 172
Main Authors Qiu, Wen-Cai, Wang, Zhi-Gang, Wang, Wei-Gang, Zheng, Qi
Format Journal Article
LanguageChinese
Published China 01.03.2008
Subjects
Animals
Female
Gastric Emptying - drug effects
Ghrelin - pharmacology
Male
Mice
Mice, Inbred C57BL
Oligopeptides - pharmacology
Stomach - drug effects
Stomach - physiology
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Summary:To investigate the effect and mechanism of ghrelin and its synthetic peptide GHRP-6 on gastric motor in mice. In vivo, the dose-dependent effects of ghrelin (20,50,100,200 mug/kg) and GHRP-6 (20,50,100,200 mug/kg) on gastric emptying were measured by intragastric application of phenol red test which was adapted for use in mice. The effects of atropine, NG-nitro-L-arginine methyl ester (L-NAME), and D-Lys(3)-GHRP-6 (GHS-R antagonist) on the gastric motor induced by ghrelin and GHRP-6 (100 mug/kg) were also investigated. In vitro, the effects of ghrelin (0.01,0.1,1.0,10.0 mumol/L) and GHRP-6 (0.01,0.1,1.0,10.0 mumol/L) on spontaneous contraction of mice fundic muscle strips were studied as well. Both ghrelin (50,100,200 mug/kg) and GHRP-6 (50,100,200 mug/kg) significantly accelerated gastric emptying (P<0.05), but they failed to accelerate gastric emptying in the presence of atropine, L-NAME and D-Lys(3)-GHRP-6 (P<0.05). Ghrelin (0.1, 1.0, 10.0 mumol/L) and GHRP-6 (0.1, 1.0, 10.0 mumol/L) induced significant co
ISSN:1671-0274