Protective effect of pyrrolidine dithiocarbamate on erythrocytes during canine cardiopulmonary bypass

• Published in: Zhōngguó yīxué kēxuéyuàn xuébào Vol. 30; no. 2; p. 165
• Main Authors: Fang, Hua, Liu, Jin, Li, Chang-Xi, Wang, Quan-Yun, Zuo, Yun-Xia
• Format: Journal Article
• Language: Chinese
• Published: China 01.04.2008
• Subjects: Animals; Cardiopulmonary Bypass - methods; Dogs; Erythrocytes - drug effects; Erythrocytes - metabolism; Hemoglobins - metabolism; Interleukin-1beta - blood; Interleukin-8 - blood; Lipid Peroxidation - drug effects; Malondialdehyde - blood; Pyrrolidines - therapeutic use; Random Allocation; Thiocarbamates - therapeutic use
• ISSN: 1000-503X

To investigate the protective effect of pyrrolidine dithiocarbamate (PDTC) on erythrocytes during canine cardiopulmonary bypass (CPB). Twelve adult healthy dogs undergoing CPB were randomly divided into the control group (n = 6) and the PDTC group (n = 6). In the PDTC group, PDTC 30 mg/kg was administered intravenously before CPB. Dogs in the control group was intravenously administering with normal saline. The levels of interleukin (IL)-1beta, IL-8, malondiadehyde (MDA), free hemoglobin (F-HB) in plasma, erythrocyte adenosine triphosphate (E-ATP), and erythrocyte superoxide dismutase (E-SOD) were determined before CPB, 30 and 60 minutes after aortic cross-clamping (AC), and 30 and 60 minutes after declamping (DC). In the control group, plasma levels of IL-1beta and IL-8 significantly increased after CPB (P < 0.01). In the PDTC group, plasma levels of IL-1beta and IL-8 significantly increased after CPB (P < 0.05, P < 0.01). Plasma levels of MDA and F-HB significantly increased (P < 0.01) and the E-ATP level a