Human osteoblasts attachment to guided tissue regeneration membranes which were coated either with platelet-rich plasma or platelet-poor plasma

To determine whether human alveolar bone osteoblasts (HABO) attachment to commercial available guided tissue regeneration (GTR) membranes can be enhanced by coating with freshly prepared human platelet-rich plasma (PRP) and platelet poor plasma (PPP). Human osteoblasts established from tissue explan...

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Bibliographic Details
Published inZhonghua kouqiang yixue zazhi Vol. 42; no. 8; p. 496
Main Authors Xu, Yan, Jiang, Yong, Lin, Xiao, Bartold, P M, V, Marino
Format Journal Article
LanguageChinese
Published China 01.08.2007
Subjects
Cell Adhesion
Cells, Cultured
Culture Media
Humans
Osteoblasts - cytology
Platelet-Rich Plasma
Tooth Socket - cytology
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Summary:To determine whether human alveolar bone osteoblasts (HABO) attachment to commercial available guided tissue regeneration (GTR) membranes can be enhanced by coating with freshly prepared human platelet-rich plasma (PRP) and platelet poor plasma (PPP). Human osteoblasts established from tissue explants were used at 4 th passage in culture. Human whole blood from healthy subjects was collected and centrifuged twice to produce the PRP fraction and PPP fraction. Double-sided adhesive tape was used to fix 3 mm discs of each membrane and cover-slides to the bottom of a 24-well tissue culture plate. A (GoreTex-ePTFE), B (GoreTex-Resolut) and C (Inion-GTR) membranes were studied. Cover-slides were positive control. Membranes or cover-slides were exposed to PRP, PPP or PBS respectively for 2 hours. Membranes and cover-slides were seeded with osteoblasts (5 x 10(7) cells/L) and allowed to attach for 24 hours. After staining with hematoxylin, the number of attached cells per mm(2) was counted using a light microscope wi
ISSN:1002-0098