Effect of rapamycin on proliferation, apoptosis and regulation of chemokine receptor CXCR4 in RPMI8226 cells

This study was purposed to investigate the effect of rapamycin on proliferation, apoptosis, cell cycle progression and the regulation of chemokine receptor CXCR4 on RPMI8226 cells. Different concentrations of rapamycin were used to treat the multiple myeloma cell line RPMI8226 for different times. T...

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Bibliographic Details
Published inZhongguo shi yan xue ye xue za zhi Vol. 17; no. 2; p. 385
Main Authors Shi, Jia-Jia, Jia, Xiao-Hui, Li, Xiao-Hong, Cheng, Zhi-Yong, Wei, Xiao-Xuan, Sun, Li-Hong, Liu, Ze-Lin
Format Journal Article
LanguageChinese
Published China 01.04.2009
Subjects
Apoptosis - drug effects
Cell Line, Tumor
Cell Proliferation - drug effects
Down-Regulation
Humans
Receptors, CXCR4 - metabolism
Sirolimus - pharmacology
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Summary:This study was purposed to investigate the effect of rapamycin on proliferation, apoptosis, cell cycle progression and the regulation of chemokine receptor CXCR4 on RPMI8226 cells. Different concentrations of rapamycin were used to treat the multiple myeloma cell line RPMI8226 for different times. The proliferation of the cells was detected by MTT assay; the apoptosis rate and cell cycle were determined by flow cytometry (FCM); apoptosis of cells was observed by inverted microscopy; the cylin D1, CXCR4 and mTOR mRNA expressions were detected by RT-PCR or FQ-PCR after treating RPMI8226 cells with different concentrations of rapamycin. The results indicated that the rapamycin could inhibit the proliferation of RPMI8226 cells and induce their apoptosis. The cell cycle was arrested at the G(0)/G(1) phase. PCR results showed the down-regulation of mTOR, cyclin D1 and mTOR mRNA expressions after treating RPMI8226 cells with different concentrations of rapamycin for 24 hours. It is concluded that the rapamycin signi
ISSN:1009-2137