Relationship between variation of coxsackievirus B3 VP1 sequence from cerebrospinal fluid of children and severity of damage to central nervous system

• Published in: Zhonghua er ke za zhi Vol. 48; no. 4; p. 268
• Main Authors: Chen, Zong-bo, Fu, Zhen-rong, Wu, Fu-ling, Sui, Ai-hua, Yang, Kun, Liu, Xiao-mei, Qian, Na, Zhao, Na, Chen, Zhen-zhen
• Format: Journal Article
• Language: Chinese
• Published: China 01.04.2010
• Subjects: Amino Acid Sequence; Base Sequence; Capsid Proteins - cerebrospinal fluid; Capsid Proteins - genetics; Central Nervous System - pathology; Central Nervous System - virology; Child; Coxsackievirus Infections - cerebrospinal fluid; Coxsackievirus Infections - epidemiology; Coxsackievirus Infections - virology; Encephalitis - virology; Enterovirus B, Human - genetics; Enterovirus B, Human - pathogenicity; Female; Humans; Male; Molecular Sequence Data; RNA, Viral - genetics; Virulence
• ISSN: 0578-1310

To investigate the possible relationship between variation of coxsackievirus B3 (CoxB3) VP1 sequence from cerebrospinal fluid of children with severe and mild central nervous system (CNS) infection and damage to CNS in children from Shandong province. The enteroviruses were detected using VP1 typing and sequencing primer for enteroviruses from 73 enterovirus-infected cases confirmed by detection of cerebrospinal fluid by enteroviruses common primer. VP1 sequences (450 nucleotides) were determined and analyzed for 21 CoxB3 enteroviruses strains isolated in Qingdao and Binzhou, and were compared with that of BLAST search procedures from GeneBank in NCBI. The variation of VP1 gene and amino acids sequence of CoxB3 enteroviruses was analyzed for severe and mild CNS infection. The nucleotide homogeneity of these CoxB3 appeared to be 97% - 99%, however, the homogeneity among different genotypes were 83% - 76%. Replacement of glutamine by histidine at amino acid locus 856 of VP1 CoxB3 was found in 4 cases with sever