The influence of JNK inhibitor on hemodynamics after ischemia/reperfusion injury to the heart in rats

• Published in: Zhongguo wei zhong bing ji jiu yi xue Vol. 20; no. 12; p. 727
• Main Authors: Qian, Hong-Jin, Li, Zhi-Liang, Jiao, Bao-Ming, Zheng, Jian-Sheng, Su, Lei
• Format: Journal Article
• Language: Chinese
• Published: China 01.12.2008
• Subjects: Animals; Anthracenes - pharmacology; Disease Models, Animal; Hemodynamics - drug effects; JNK Mitogen-Activated Protein Kinases - antagonists & inhibitors; Male; Myocardial Reperfusion Injury - drug therapy; Myocardial Reperfusion Injury - physiopathology; Protein Kinase Inhibitors - pharmacology; Pyrazines - pharmacology; Random Allocation; Rats; Rats, Sprague-Dawley
• ISSN: 1003-0603

To investigate the effect and mechanism of JNK mitogen-activated protein kinases (JNK MAPKs) inhibitor SP 600125 on hemodynamics after ischemia/reperfusion (I/R) injury to heart in anesthetized rats. Thirty-six healthy adult Sprague-Dawley (SD) rats were randomly divided into six groups (each n=6): sham operation (SO) group, I/R group, three JNK inhibitor groups (model groups) and ligustrazine hydrochloride (LH) group. In the SO group, a silk suture was passed underneath a main branch of the left coronary artery without tying. In the rest groups, the left coronary artery was occluded lasting for 30 minutes followed by reperfusion for 180 minutes. In the model groups, SP 600125 was intravenously administered 5 minutes before the end of the ischemia period, and continued during reperfusion period with a total dose of 4.7, 14.4 and 47.9 mg/kg respectively. Control animals received normal saline or LH 30 mg/kg in the same manner. The changes in hemodynamics, including heart rate (HR), mean blood pressure (MBP), m