Genome DNA hypomethylation in the process of crystalline nickel-induced cell malignant transformation

• Published in: Zhōnghuá yùfáng-yīxué zázhì Vol. 44; no. 7; p. 622
• Main Authors: Yang, Lin-qing, Ji, Wei-dong, Tao, Gong-hua, Zhang, Wen-juan, Gong, Chun-mei, Zhou, Li, Liu, Jian-jun, Ke, Yue-bin, Zhuang, Zhi-xiong
• Format: Journal Article
• Language: Chinese
• Published: China 01.07.2010
• Subjects: Bronchi - cytology; Cell Line; Cell Transformation, Neoplastic - chemically induced; DNA Methylation - drug effects; Epithelial Cells - drug effects; Genome; Humans; Nickel - adverse effects; Nickel - chemistry
• ISSN: 0253-9624

To observe the effect of crystalline NiS on genome DNA methylation profile in in vitro cultured cells. 16HBE Cells were treated with crystalline NiS at 0.25, 0.50, 1.00 and 2.00 µg/cm(2) for 24 h and three times at total. DAC treatment was given at 3 µmol/L for 72 h.5-mC immunofluorescence and SssI methyltransferase assay methods were applied to investigate if the hypomethylation of genome DNA involved. The results of 5-mC immunofluorescence showed that the fluorescence intensity of NiS-treated cells were decreased in some degree, and transformed cells were decreased dramatically. By the SssI methylase assay, an average of (81.9 ± 7.3)% methylated CpG were found in negative control cells. By contrast, (77.9 ± 6.2)%, (75.3 ± 6.8)%, (59.5 ± 4.9)%, (67.4 ± 5.1)% methylated CpG were observed in cells treated with NiS for three times at dosage of 0.25, 0.50, 1.00 and 2.00 µg/cm(2) which were abbreviated as NiS0.25, NiS0.50, NiS1.00, NiS2.00 respectively. The ANOVA analysis results showed that there was a significa