Influence of cytokine combinations on proliferation and differentiation of umbilical cord blood CD34(+) cells into megakaryocytes/platelets in vitro

• Published in: Zhongguo shi yan xue ye xue za zhi Vol. 19; no. 4; p. 1053
• Main Authors: Zhang, Ke-Ying, Liu, Jiang, Jia, Yan-Jun, Li, Wei, Duan, Lan, Gao, Song-Ming, Cui, Shuang, Gong, Zhi-Yin, Ni, Lei, Zhang, Zhi-Xin
• Format: Journal Article
• Language: Chinese
• Published: China 01.08.2011
• Subjects: Antigens, CD34 - immunology; Blood Platelets - cytology; Cell Differentiation; Fetal Blood - cytology; Fetal Blood - immunology; Humans; Interleukin-11 - pharmacology; Interleukin-3 - pharmacology; Megakaryocytes - cytology; Stem Cell Factor - pharmacology; Thrombopoietin - pharmacology
• ISSN: 1009-2137

In order to investigate the influence of cytokine combinations on proliferation and differentiation of human umbilical cord blood CD34(+) cells into megakaryocytes/platelets in vitro, the CD34(+) cells from human umbilical cord blood were amplified in serum-free medium StemSpan(SFEM) supplemented with several cytokine combinations by three-phase culture system. The effects of the cytokine combinations were compared. The results showed that at day 14 of the first culture phase, the CD34(+) cells cultured with cytokine combinations SCF + TPO + FL + IL-3 were amplified (11 000 ± 1 000) times, which were significantly higher than that of cells cultured with SCF + TPO + FL, but were not significantly different from that of cells cultured with SCF + TPO + IL-3 or SCF + TPO + FL + IL-3+ hydroxyl-corticosteroids. At day 7 of the second culture phase, the CD34(+) cells cultured with cytokine combination SCF + TPO + FL + IL-11 were amplified by (204666.7 ± 11718.9) times, which were significantly higher than that of ce