Inhibitory effects of pretreatment with Buyanghuanwu decoction on inflammatory cytokine expressions in the kidneys of rats after induction of brain death

• Published in: Zhong yao cai Vol. 32; no. 12; p. 1855
• Main Authors: Chen, Jie, Zeng, Yao-ying, Zeng, Hui-lan, Chen, Xiao-yin, Su, Ze-xuan, Liu, Jia-wen, Wang, Qiao-feng, Li, Wei, Wu, Wen-yan
• Format: Journal Article
• Language: Chinese
• Published: China 01.12.2009
• Subjects: Animals; Brain Death; Disease Models, Animal; Drug Combinations; Drugs, Chinese Herbal - isolation & purification; Drugs, Chinese Herbal - pharmacology; Interleukin-1 - genetics; Interleukin-1 - metabolism; Kidney - drug effects; Kidney - metabolism; Kidney - pathology; Male; Neutrophils - metabolism; p38 Mitogen-Activated Protein Kinases - metabolism; Plants, Medicinal - chemistry; Random Allocation; Rats; Rats, Wistar; Reactive Oxygen Species - metabolism; Reverse Transcriptase Polymerase Chain Reaction; RNA, Messenger - genetics; RNA, Messenger - metabolism; Tumor Necrosis Factor-alpha - genetics; Tumor Necrosis Factor-alpha - metabolism
• ISSN: 1001-4454

To observe the inhibitiory effects of pretreatment with Buyanghuanwu decoction (BYHWT) on inflammatory cytokine expressions in the kidneys and the level of reactive oxygen species (ROS) by peripheral blood neutrophils of rats after induction of brain death (BD), and to investigate the effect of BYHWT on the improvement of kidney quality from BD donor. 30 male Wistar rats were randomly divided into 3 groups: control group, BD model group and BYHWT group. 6 hours after successful onset of brain death,only the BD rats whose mean arterial blood pressure were higher than 80 mmHg were accepted as donors. Kidneys were harvested and peripheral blood was taken from BD rats. RT-PCR was used to detect the expressions of TNF-a and IL-lpfl mRNA. Western blot was adopted to analyze the expressions of both TNF-alpha and IL-lp protein,and the expression of phosphorylated p38 mitogen-activated protein kinase (p-p38MAPK). Reactive oxygen species( ROS) in the peripheral blood neutrophils were labeled with CM-H2DCFDA and then de