Application of laser capture microdissection and surface-enhanced laser desorption ionization time-of-flight mass spectrometry to screen biomarkers for early diagnosis of lung adenocarcinoma

• Published in: Sichuan da xue xue bao. Journal of Sichuan University. Yi xue ban Vol. 40; no. 1; p. 157
• Main Authors: Tian, Ying-Xuan, Yang, Shuan-Ying, Nan, Yan-Dong, Zhang, Wei, Yu, Jie-Kai, Zheng, Shu
• Format: Journal Article
• Language: Chinese
• Published: China 01.01.2009
• Subjects: Adenocarcinoma - diagnosis; Adenocarcinoma - metabolism; Aged; Biomarkers, Tumor - analysis; Early Diagnosis; Female; Humans; Lasers; Lung Neoplasms - diagnosis; Lung Neoplasms - metabolism; Male; Microdissection - methods; Middle Aged; Neoplasm Proteins - analysis; Sensitivity and Specificity; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - methods
• ISSN: 1672-173X

To improve diagnostic methods to screen biomarkers for early diagnosis in lung adenocarcinoma by employing laser capture microdissection (LCM) and surface-enhanced laser desorption ionization time-of-flight mass spectrometry (SELDI-TOF-MS) and support vector machine (SVM). Frozen sections of thickness 8 microm were made using 6 cases of fresh lung cancer tissues and 4 cases of matched normal lung tissues. The sections were stained by improved HE solution. The homogeneous adenocarcinoma cells and normal cells were collected by LCM in each sample, and then SELDI profiles based on PBS II+SELDI-TOF-MS (IMAC protein chip) were analyzed using SVM. High quality cell samples were obtained by LCM quickly and precisely from normal specimens and diseased tissues without interstitium, inflammation and necrosis. Eighty four differential protein peaks were found. Top ten of them were identified as candidate biomarkers; six proteins were significantly weakly expressed in lung cancer tissue compared to normal tissues, but th