Effects of Huoxuezhitong recipe on the pathway of mitochondrial apoptosis and the expression of related gene in epidural scar tissue

• Published in: Zhongguo gu shang Vol. 23; no. 10; p. 776
• Main Authors: Fan, Xi-Ling, Zhou, Hui, Xia, Zhi-Min, Niu, Hai-Xin, Luo, Jun-Jun
• Format: Journal Article
• Language: Chinese
• Published: China 01.10.2010
• Subjects: Actins - genetics; Actins - metabolism; Animals; Apoptosis - drug effects; Apoptosis - genetics; bcl-2-Associated X Protein - genetics; bcl-2-Associated X Protein - metabolism; Carthamus tinctorius; Caspase 3 - genetics; Caspase 3 - metabolism; Caspase 9 - genetics; Caspase 9 - metabolism; Drugs, Chinese Herbal - pharmacology; Female; Genes, bcl-2 - drug effects; Genes, bcl-2 - genetics; Mitochondria - drug effects; Mitochondria - pathology; Proto-Oncogene Proteins c-bcl-2 - genetics; Proto-Oncogene Proteins c-bcl-2 - metabolism; Rabbits; Reverse Transcriptase Polymerase Chain Reaction; RNA, Messenger - metabolism
• ISSN: 1003-0034

To study the effect of Huoxuezhitong recipe on the pathway of mitochondrial apoptosis and the expression of related gene (Caspase-3, -9, Bax, Bcl-2) in epidural scar tissue. A total of 60 New Zealand rabbits, ranging in weight from 2.5 to 3.0 kg, were randomly divided into sham operative group (group A), control group (group B), sodium hyaluronate group (group C), Huoxuezhitong recipe group (group D, included Danggui 20 g, Chishao 20 g, Honghua 20 g, Ruxiang 15 g, Myrrh 15 g etc.) with 15 rabbits in each group. Except for group A, L4,5 vertebral plate were resected leading to 1.0 cm x 1.0 cm dura mater exposed area, which were covered with 0.5 ml sodium hyaluronate in group C, covered with same amount of saline in group B and D. At 2 weeks after operation, the rabbits of group D were administered with Huoxuezhitong recipe (2.5 ml/kg, once a day, continuous 14 d). Five rabbits of each group were selected randomly and were killed at 2, 4 and 8 weeks after operation; and the expression of Caspase-3, -9, Bax, Bcl