Expression features of glypican-3 and its diagnostic and differential values in hepatocellular carcinoma

• Published in: Zhonghua gan zang bing za zhi Vol. 19; no. 4; p. 260
• Main Authors: Bian, Yin-zhu, Yao, Deng-fu, Zhang, Chong-guo, Li, Shan-shan, Wu, Wei, Dong, Zhi-zhen, Qiu, Li-wei, Yu, Dan-dan
• Format: Journal Article
• Language: Chinese
• Published: China 01.04.2011
• Subjects: Adolescent; Adult; Aged; Aged, 80 and over; Animals; Carcinoma, Hepatocellular - diagnosis; Carcinoma, Hepatocellular - metabolism; Carcinoma, Hepatocellular - pathology; Diagnosis, Differential; Female; Glypicans - metabolism; Humans; Liver - pathology; Liver Neoplasms - diagnosis; Liver Neoplasms - metabolism; Liver Neoplasms - pathology; Male; Middle Aged; Rats; Rats, Sprague-Dawley; Young Adult
• ISSN: 1007-3418
• DOI: 10.3760/cma.j.issn.1007-3418.2011.04.008

To investigate the expression features of glypican-3 (GPC-3) and its diagnostic and differential values in hepatocellular carcinoma (HCC). Rat hepatoma models were made and the dynamic expression features of GPC-3 protein and its gene were investigated by Western blotting and RT-PCR respectively. Liver specimens from 36 HCC patients were collected by self-control method and the expression and clinicopathological features of GPC-3 were analyzed by immunohistochemistry. Serum GPC-3 levels were quantitatively detected by ELISA and its efficiency for HCC diagnosis was evaluated in patients with liver diseases. The incidence of GPC-3 was 0% in control, 83.3% in degeneration, 100% in precancerosis and 100% in canceration during dynamic formation of rat hepatoma, respectively. The positive GPC-3 was brown granule- like staining localized in membrane and cytoplasm in human HCC. The GPC-3 positive rates were 80.6% in HCC, 41.7% in surrounding tissues and none in distal tissues (P < 0.01), respectively. No positive rel