Exogenous hydrogen sulfide attenuates gastric ischemia-reperfusion injury via activation of K(ATP) channel

• Published in: Sheng li hsüeh pao Vol. 64; no. 1; p. 27
• Main Authors: Zou, Ji-He, Qiao, Wei-Li, Wang, Guang-Ming, Ma, Hong-Ji, Qi, You-Jian, Sun, Hong, Yan, Chang-Dong
• Format: Journal Article
• Language: Chinese
• Published: China 25.02.2012
• Subjects: Animals; Gastric Mucosa - pathology; Hydrogen Sulfide - metabolism; Ischemic Preconditioning - methods; KATP Channels - metabolism; KATP Channels - physiology; Male; Rats; Rats, Sprague-Dawley; Reperfusion Injury - prevention & control; Stomach - blood supply; Sulfides - pharmacology
• ISSN: 0371-0874

The present study aimed to investigate the protective effect and mechanism of hydrogen sulfide donor NaHS administration against gastric mucosal injury induced by gastric ischemia-reperfusion (GI-R) in rats. GI-R injury was induced by clamping the celiac artery of adult male SD rats for 30 min and followed by reperfusion for 1 h. The rats were randomly divided into sham group, GI-R group, NaHS group, glibenclamide group and pinacidil group. Gastric mucosal damage was analyzed with macroscopic injured area, deep damage was assessed with histopathology scores, and the hydrogen sulfide concentration in plasma was determined by colorimetric method. The results showed that pretreatment of NaHS significantly reduced the injured area and deep damage of the gastric mucosa induced by GI-R. However, NaHS did not significantly alter the levels of hydrogen sulfide in plasma 14 d after NaHS administration. The gastric protective effect of NaHS during reperfusion could be attenuated by glibenclamide, an ATP-sensitive potas