Imaging, autoradiography, and biodistribution of (188)Re-labeled PEGylated nanoliposome in orthotopic glioma bearing rat model

• Published in: Cancer biotherapy & radiopharmaceuticals Vol. 26; no. 6; p. 717
• Main Authors: Huang, Feng-Yun J, Lee, Te-Wei, Kao, Chih-Hao K, Chang, Chih-Hsien, Zhang, Xiaoning, Lee, Wan-Yu, Chen, Wan-Jou, Wang, Shu-Chi, Lo, Jem-Mau
• Format: Journal Article
• Language: English
• Published: United States 01.12.2011
• Subjects: Animals; Autoradiography - methods; Brain Neoplasms - diagnostic imaging; Brain Neoplasms - metabolism; Cell Line, Tumor; Disease Models, Animal; Glioma - diagnostic imaging; Glioma - metabolism; Isotope Labeling - methods; Liposomes - chemistry; Liposomes - pharmacokinetics; Male; Nanoparticles - chemistry; Radioisotopes - chemistry; Radioisotopes - pharmacokinetics; Radiopharmaceuticals - pharmacokinetics; Rats; Rhenium - chemistry; Rhenium - pharmacokinetics; Tissue Distribution; Tomography, Emission-Computed - methods; Tomography, Emission-Computed, Single-Photon - methods
• ISSN: 1557-8852
• DOI: 10.1089/cbr.2011.1052

The (188)Re-labeled pegylated nanoliposome (abbreviated as (188)Re-Liposome) was prepared and evaluated for its potential as a theragnostic agent for glioma. (188)Re-BMEDA complex was loaded into the pegylated liposome core with pH 5.5 ammonium sulfate gradient to produce (188)Re-Liposome. Orthotopic Fischer344/F98 glioma tumor-bearing rats were prepared and intravenously injected with (188)Re-Liposome. Biodistribution, pharmacokinetic study, autoradiography (ARG), histopathology, and nano-SPECT/CT imaging were conducted for the animal model. The result showed that (188)Re-Liposome accumulated in the brain tumor of the animal model from 0.28%±0.09% injected dose (ID)/g (n=3) at 1 hour to a maximum of 1.95%±0.35% ID/g (n=3) at 24 hours postinjection. The tumor-to-normal brain uptake ratio (T/N ratio) increased from 3.5 at 1 hour to 32.5 at 24 hours. Both ARG and histopathological images clearly showed corresponding tumor regions with high T/N ratios. Nano-SPECT/CT detected a very clear tumor image from 4 hours till 48 hours. This study reveals the potential of (188)Re-Liposome as a theragnostic agent for brain glioma.