Colloidal gold and dot-ELISA rapid tests for screening influenza A virus

• Published in: Nan fang yi ke da xue xue bao = Journal of Southern Medical University Vol. 30; no. 10; p. 2267
• Main Authors: Jia, Ning, Yan, Zhong-qiang, Liu, Gang, Shen, Ding-xia, Suo, Ji-jiang, Xing, Yu-bin, Gao, Yan, Liu, Yun-xi
• Format: Journal Article
• Language: Chinese
• Published: China 01.10.2010
• Subjects: Enzyme-Linked Immunosorbent Assay; Gold Colloid; Humans; Influenza A Virus, H1N1 Subtype; Influenza, Human - diagnosis; Influenza, Human - virology; Real-Time Polymerase Chain Reaction; Sensitivity and Specificity
• ISSN: 1673-4254

To evaluate the diagnostic efficiency of colloidal gold and dot ELISA rapid tests in clinical screening of influenza A virus. The pharyngeal swabs were collected from 297 outpatients suspected of influenza between June and October, 2009 for detection with colloid gold and dot ELISA rapid test, with real-time PCR as the golden methods. The discrepant results of colloid gold and dot ELISA methods were confirmed by sequencing, and the diagnostic efficiency of the two methods was evaluated. Among the 166 samples with influenza A virus infection as confirmed by real-time PCR and sequencing, the diagnostic sensitivity of dot ELISA and colloid gold methods was 54.82% (91/166) and 4.22% (7/166), respectively. The total concordance rate with PCR was 66.67% (Kappa value of 0.35). Among the 133 samples negative for influenza A virus, the specificity of dot ELISA and colloid gold methods was 81.68% (107/131) and 98.47% (129/131), respectively, with a total concordance rate with PCR of 45.79% (Kappa value 0.02). Of the 99