The effects of vitamin E and dexamethasone on inflammation of acute lung injury and expression of myosin light chain kinase

• Published in: Zhonghua jiehe he huxi zazhi Vol. 32; no. 1; p. 46
• Main Authors: Bai, Jian-Wen, Deng, Wei-Wu, Lin, Min-Jia, Xu, Shu-Min, Zhang, Dou-Xia
• Format: Journal Article
• Language: Chinese
• Published: China 01.01.2009
• Subjects: Acute Lung Injury - metabolism; Acute Lung Injury - pathology; Animals; Dexamethasone - pharmacology; Female; Inflammation; Lung - drug effects; Lung - metabolism; Lung - pathology; Mice; Mice, Inbred BALB C; Myosin-Light-Chain Kinase - metabolism; Vitamin E - pharmacology
• ISSN: 1001-0939

To observe the effect of vitamin E (VitE) combined with dexamethasone (DXM) on inflammation of acute lung injury and expression of myosin light chain kinase. Forty female Balb/c mice were randomly divided into 4 groups, a saline control group (1.5 ml/kg), a LPS group (1 mg/kg), a VitE and DXM group (VitE 50 mg/kg, DXM 1 mg/kg), and a VitE group (50 mg/kg). Lung tissue histopathological changes were observed. Immunohistochemistry assays (SABC) were used to determine the myosin light chain kinase (MLCK) immunoreactive cells in the lung tissues, and the MLCK mRNA and the MLCK protein was assayed by RT-PCR and by Western blot, respectively. Means were compared with analysis of variance and Student-Newman-Keuls were used to compare 2 means. Histological examination showed that extensive lung inflammation were seen in the LPS group, which manifested by accumulation of significant numbers of neutrophils, accompanied by marked pulmonary edema and hemorrhage. The inflammation and hemorrhage in the 2 treatment groups w