A indirect competitive ELISA to detect domoic acid in seawater and shellfish

A competitive enzyme-linked immunosorbent assay (ELISA) was developed for measurent domoic acid (DA) in seawater and shellfish. DA was coupled to bovine serum albumin (BSA) and ovalbumin (OVA) by carbodiimide reaction. DA-BSA as immunogen was injected in BALB/c mice. Titres of the antisera against D...

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Bibliographic Details
Published inWei sheng yan jiu Vol. 38; no. 5; p. 622
Main Authors Liu, Renyan, Xu, Daoyan, Dong, Yuhua, Liang, Yubo
Format Journal Article
LanguageChinese
Published China 01.09.2009
Subjects
Animals
Enzyme-Linked Immunosorbent Assay - methods
Female
Kainic Acid - analogs & derivatives
Kainic Acid - analysis
Kainic Acid - immunology
Marine Toxins - analysis
Mice
Mice, Inbred BALB C
Neurotoxins - analysis
Seawater - analysis
Shellfish - analysis
Water Pollutants, Chemical - analysis
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Summary:A competitive enzyme-linked immunosorbent assay (ELISA) was developed for measurent domoic acid (DA) in seawater and shellfish. DA was coupled to bovine serum albumin (BSA) and ovalbumin (OVA) by carbodiimide reaction. DA-BSA as immunogen was injected in BALB/c mice. Titres of the antisera against DA were determinated using DA-OVA as coating ligand by ELISA method. After female BALB/c mice were immunized six times, the polyclonal antibodies anti-DA was obtained. The competitive indirect ELISA for domoic acid in shellfish and seawater was established. Under optimal condition, the detection limit of DA was 10.0 ng/ml (equal 4 microg/g shellfish meat). The recovery of domoic acid added in seawater was 83.2% - 124.7% with a coefficient of variation of 4.7% - 5.9%, the recovery of domoic acid added in shellfish was 85.9% - 99.9% with a coefficient of variation of 2.4% - 7.1%.
ISSN:1000-8020