Probing R6G single molecules by surface-enhanced resonance Raman scattering

R6G was incubated in three kinds of silver colloid with different low concentrations, and its surface-enhanced resonance Raman scattering (SERRS) was studied with a confocal microscopy system. Each sample exhibited different spectrum character. At the single-molecule level the SERRS-spectra were rec...

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Published inGuang pu xue yu guang pu fen xi Vol. 25; no. 12; p. 1986
Main Authors Zhou, Zeng-Hui, Liu, Li, Xiao, Fan-Rong, Wang, Gui-Ying, Xu, Zhi-Zhan
Format Journal Article
LanguageChinese
Published China 01.12.2005
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Summary:R6G was incubated in three kinds of silver colloid with different low concentrations, and its surface-enhanced resonance Raman scattering (SERRS) was studied with a confocal microscopy system. Each sample exhibited different spectrum character. At the single-molecule level the SERRS-spectra were recorded in 10(-13) mol x L(-1) dye colloidal solution. Some spectral inhomogeneous behavior was recorded such as spectral diffusion, intensity fluctuation of vibrational lines and spectral polarization, and even on/off blinking and splitting of some lines within the spectrum of one molecule. Polarization of excitation light and background subtraction of signal have an important influence on the SERRS spectra detection of single-molecule, which was analyzed in the present paper.
ISSN:1000-0593