Organ-specific T cell receptor repertoire in target organs of murine graft-versus-host disease after haploidentical bone marrow transplantation

• Published in: Zhongguo shi yan xue ye xue za zhi Vol. 17; no. 4; p. 999
• Main Authors: Fu, Yue-Wen, Wu, De-Pei, Chen, Feng, Feng, Yu-Feng, Song, Yong-Ping, Zhu, Ping
• Format: Journal Article
• Language: Chinese
• Published: China 01.08.2009
• Subjects: Amino Acid Sequence; Animals; Bone Marrow Transplantation; Complementarity Determining Regions - genetics; Graft vs Host Disease - genetics; Graft vs Host Disease - immunology; Immunoglobulin Variable Region - genetics; Mice; Mice, Inbred BALB C; Mice, Inbred C57BL; Molecular Sequence Data; Receptors, Antigen, T-Cell - genetics
• ISSN: 1009-2137

This study was purpused to analyze the characteristics of T cell receptor repertoire in target organs of murine graft-versus-host after haploidentical bone marrow transplantation (hiBMT) and the molecular characteristics of complementarity determining region3 (CDR3) repertoires of monoclonal T cell in liver, skin and ileum in murine after hiBMT. Murine haploidentical BMT model was established, CDR3-size spectratyping was used to study TCRBV repertoires in recipient liver, skin, ileum, spleen and a group of CDR3 molecules was obtained from GVHD-target tissues. The results showed that GVHD occurred as early as days 14 after transplantation and was proven by histology in liver, skin and ileum. A number of new monoclonal and oligoclonal T cells emerged in GVHD-target tissue. 45 CDR3 molecules had six C'-terminal motifs, which obtained from liver, skin, ileum in different times after hiBMT. It is concluded that target organs of murine graft-versus-host disease after hiBMT emerged a number of clonal or oligoclonal