Influence of derivatives of arachidonic and docosohexaenic acids on AMPA receptors in Purkinje neurons and cognitive functions in mice

• Published in: Izvestiia Akademii nauk. Seriia biologicheskaia no. 3; p. 370
• Main Authors: Grigor'ev, V V, Serkov, I V, Beznosko, B K, Ivanova, T A, Gretskaia, N M, Bezuglov, V V, Bachurin, S O
• Format: Journal Article
• Language: Russian
• Published: Russia (Federation) 01.05.2010
• Subjects: Animals; Arachidonic Acids - chemistry; Arachidonic Acids - pharmacology; Behavior, Animal - drug effects; Cells, Cultured; Cognition - drug effects; Docosahexaenoic Acids - chemistry; Docosahexaenoic Acids - pharmacology; Dose-Response Relationship, Drug; Male; Membrane Potentials - drug effects; Memory - drug effects; Mice; Mice, Inbred C57BL; Patch-Clamp Techniques; Purkinje Cells - drug effects; Purkinje Cells - metabolism; Rats; Reaction Time - drug effects; Receptors, AMPA - metabolism
• ISSN: 1026-3470

The effect of derivatives of arachidonic and docosahexaenoic acids on AMPA receptors in Purkinje cells from the rat cerebellum was studied using the patch-clamp electrophysiological method. It was shown that derivatives of arachidonic acid-arachidonoyl dopamine and docosahexaenoic acid-docosahexaenoyl dopamine and ester of docosahexaenoic acid with ethylene glycol in nanomolar concentrations effectively potentiated the ionic currents caused by activation of AMPA receptors of kainic acid. Ester of docosahexaenoic acid with nitroethylene glycol blocked AMPA receptors, and anandamide (ethanolamide of arachidonic acid) was not effective. A behavioral test showed that docosahexaenoyl dopamine in doses of 0.1-20 mg/kg had no effect on the learning and memory abilities of the animals tested.