Study of the effect of p53 and Bcl-2 protein expression in myocardial apoptosis in septic rats

• Published in: Zhongguo wei zhong bing ji jiu yi xue Vol. 20; no. 12; p. 733
• Main Authors: Zhai, Cheng-Shun, Tang, Hao, Liang, Yan-Bing, Chen, Zhi-Bin, Li, Zhen-Yu, Ma, Zhong-Fu
• Format: Journal Article
• Language: Chinese
• Published: China 01.12.2008
• Subjects: Animals; Apoptosis; Disease Models, Animal; Male; Myocardium - metabolism; Myocardium - pathology; Proto-Oncogene Proteins c-bcl-2 - genetics; Proto-Oncogene Proteins c-bcl-2 - metabolism; Random Allocation; Rats; Rats, Sprague-Dawley; Sepsis - metabolism; Sepsis - pathology; Tumor Suppressor Protein p53 - genetics; Tumor Suppressor Protein p53 - metabolism
• ISSN: 1003-0603

To explore the significance of p53 and Bcl-2 gene in myocardial apoptosis in septic rats. Adult male Sprague-Dawley (SD) rats were randomly divided into normal control group (n=6), sham operation group (n=6) and cecal ligation and puncture (CLP) group (n=24). The sepsis model was reproduced by CLP. Rats in CLP group were divided into four subgroups according to the time points of 3, 9, 12, and 24 hours after operation, with 6 rats in each subgroup, and their hearts were examined according to the experimental protocol. Myocardial apoptosis was detected with electron microscopy and terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) technique. Bcl-2 and p53 protein expression was measured by immunohistochemistry method. Myocardial apoptosis index (AI) in septic rats were increased, higher than that in normal control group [(1.500+/- 0.141)%] and sham operation group [(1.567+/-0.258)%, all P<0.05]. The myocardial AI peaked at 12 hours [(55.633+/-2.073)%], and lowered at 24 hours [(33.68