Susceptibility of chromosomal damage among workers exposed to vinyl chloride monomer

To explore the association between chromosomal damage induced by vinyl chloride monomer (VCM) and polymorphisms of xenobiotic metabolism genes and DNA repair genes. Cytokinesis-block micronucleus (CBMN) test was performed to detect chromosomal damage in peripheral lymphocytes of 402 VCM-exposed work...

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Published inZhonghua laodong weisheng zhiyebing zazhi Vol. 29; no. 8; p. 583
Main Authors Ji, Fang, Zheng, Ying-Jia, Wang, Qi, Wang, Wei, Qiu, Yu-Lan, Wu, Fen, Chai, Shang-Jian, Li, Jun, Xia, Zhao-Lin
Format Journal Article
LanguageChinese
Published China 01.08.2011
Subjects
Adult
Chromosome Aberrations
DNA Damage
DNA Repair
Female
Genetic Predisposition to Disease
Genotype
Humans
Male
Micronucleus Tests
Middle Aged
Occupational Exposure
Vinyl Chloride - toxicity
Young Adult
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Summary:To explore the association between chromosomal damage induced by vinyl chloride monomer (VCM) and polymorphisms of xenobiotic metabolism genes and DNA repair genes. Cytokinesis-block micronucleus (CBMN) test was performed to detect chromosomal damage in peripheral lymphocytes of 402 VCM-exposed workers. Multiplex PCR was used to simultaneously amplify GSTM1 and GSTT1 genes, other genetic polymorphisms were performed using a PCR-RFLP technique. Multiple (adjusted) Poisson regression analysis showed that mean MN frequencies were significantly elevated for the intermediate (4000-40000 mg) and high (> 40000 mg) exposure groups as compared with the low exposure group (P = 0.003 and 0.03, respectively). For genetic polymorphisms, the exposed workers with CYP2E1 or XRCC1 Arg280His variance showed a higher CBMN frequency than their wild-type homozygous counterparts (P = 0.02); so did the workers with GSTP1 105Val/Val genotype or ALDH2 504Glu/Glu genotype than those with a combination of other genotypes (P = 0.01 and
ISSN:1001-9391