Genetic polymorphisms at five STR loci in Chengdu Han population

To understand the allele structure and genetic polymorphisms at five STR loci in Chengdu Han population, to obtain a preliminary database and to analyze the value in forensic medicine. EDTA-blood specimens were collected from unrelated individuals. The DNA of samples was extracted with Chelex-100 me...

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Published inSichuan da xue xue bao. Journal of Sichuan University. Yi xue ban Vol. 38; no. 2; p. 272
Main Authors Chen, Xiao, Song, Yan-Hua, Li, Ying, Liao, Miao, Long, Bin, Chen, Guo-Di
Format Journal Article
LanguageChinese
Published China 01.03.2007
Subjects
Asian Continental Ancestry Group - ethnology
Asian Continental Ancestry Group - genetics
China - ethnology
Electrophoresis
Ethnic Groups - genetics
Female
Gene Frequency
Genetic Loci - genetics
Genotype
Humans
Male
Microsatellite Repeats - genetics
Polymorphism, Genetic - genetics
China
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Summary:To understand the allele structure and genetic polymorphisms at five STR loci in Chengdu Han population, to obtain a preliminary database and to analyze the value in forensic medicine. EDTA-blood specimens were collected from unrelated individuals. The DNA of samples was extracted with Chelex-100 method. And then the PCR amplification, PAGE horizontal electrophoresis and gel silver staining were performed for analyzing the polymorphisms of five STR loci in Chengdu Han population. In the five STR loci,10, 7, 5, 7 and 7 alleles were found respectively. The observed heterozygosity of 83%, 80%, 64%, 66% and 77% and discrimination power of 95.44%, 91.0%, 84.3%, 86.1% and 86.8% were identified for the five STR loci respectively. All loci obeyed Hardy-Weinberg equilibrium. The five STR loci display the high discrimination power. The obtained data are desirable for forensic analysis and benefiting to the research of the population genetics.
ISSN:1672-173X