p35 and p25 expressions and Cdk5 kinase activity in primary cultured rat hippocampal neurons with X-ray exposure

• Published in: Nan fang yi ke da xue xue bao = Journal of Southern Medical University Vol. 29; no. 3; p. 405
• Main Authors: Han, Yong-Qing, Sun, Ai-Min, Liu, Que-Ling, Chen, Long-Hua, Yuan, Ya-Wei
• Format: Journal Article
• Language: Chinese
• Published: China 01.03.2009
• Subjects: Animals; Animals, Newborn; Cells, Cultured; Cyclin-Dependent Kinase 5 - genetics; Cyclin-Dependent Kinase 5 - metabolism; Female; Hippocampus - cytology; Hippocampus - metabolism; Hippocampus - radiation effects; Male; Neurons - cytology; Neurons - metabolism; Neurons - radiation effects; Phosphotransferases - genetics; Phosphotransferases - metabolism; Rats; Rats, Sprague-Dawley
• ISSN: 1673-4254

To study the expressions of p35 and p25 and Cdk5 kinase activity in cultured rats hippocampal neurons following X-ray exposure to provide experimental evidence for prevention and treatment of radiation encephalopathy. The hippocampal neurons cultured for 12 days were subjected to a single-dose X-ray exposure of 30 Gy. Western blotting was used to detect the p35 and p25 protein levels, and the effect of pretreatment with roscovitine, a Cdk5 inhibitor, on the apoptosis of the hippocampal neurons following the exposure was examined with 4',6-diamidino-2-phenylindole (DAPI) staining. The protein level of p35 increased significantly 3.5 and 4 h after the irradiation by 1.51-/+0.13 and 1.45-/+0.14 folds in comparison with the control level, respectively (P<0.01), and the p25 level increased significantly 6 h after irradiation by 1.62-/+0.28 folds (P<0.05). Nuclear condensation occurred in (24.8-/+3.97)% of the neurons 24 h after 30 Gy X-ray exposure, a rate significantly higher than that in the nonexposed cells [(1