Candidate gene association study of the radiosensitivity of human chromosomes with candidate gene polymorphisms upon exposure to gamma-irradiation in vitro and in vitro

The genotypic associations of the frequencies of spontaneous and radiation-induced chromosome aberrations in human lymphocytes were studied to develop genetic tests for elevated and reduced radiosensitivity. Cytogenetic analysis and genotyping (19 sites of detoxification and DNA repair genes) were c...

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Bibliographic Details
Published inGigiena i sanitarija no. 5; p. 14
Main Authors Abilev, S K, Sal'nikova, L E, Rubanovich, A V
Format Journal Article
LanguageRussian
Published Russia (Federation) 01.09.2011
Subjects
Adult
Alleles
Case-Control Studies
Chernobyl Nuclear Accident
Chromosome Aberrations - radiation effects
Chromosome Aberrations - statistics & numerical data
DNA Repair - genetics
Female
Gamma Rays - adverse effects
Gene Frequency
Genetic Association Studies
Genotype
Humans
In Vitro Techniques
Inactivation, Metabolic - genetics
Lymphocytes - radiation effects
Male
Russia
Ukraine
Xenobiotics - pharmacokinetics
Young Adult
Ukraine
Russia
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Summary:The genotypic associations of the frequencies of spontaneous and radiation-induced chromosome aberrations in human lymphocytes were studied to develop genetic tests for elevated and reduced radiosensitivity. Cytogenetic analysis and genotyping (19 sites of detoxification and DNA repair genes) were carried out for a sample of Chernobyl cleanup workers (n = 83) and for a homogenous control sample of volunteers (n = 99). In both groups, the frequency of chromosome-type aberrations proved to be elevated in carriers of minor alleles in the XPD gene (sites T2251G (Lys751Gln) and G862A (Asp312Asn)) and a combination of GSTM1-GSTT1-positive genotypes. The polymorphism of these gene did not affect the frequency of gamma-radiation-induced aberrations in the controls (1 Gy in vitro), which was associated with the alleles of the OGG1, XRCC1, and CYP1A1 genes. Thus, the frequencies of spontaneous and in vitro induced chromosome-type aberrations are associated with the alleles of different xenobiotic detoxification and DNA repair genes. At the same time, among the cleanup workers (irradiated in vivo), the elevated frequency of aberrations was observed in the carriers of the genotypes associated with the higher rate of spontaneous (but not induced in vitro) cytogenetic damages in the controls.
ISSN:0016-9900