Immune protection of tegument protein rSj29 against Schistosoma japonicum in mice

• Published in: Zhongguo jishengchongxue yu jishengchongping zazhi Vol. 27; no. 6; p. 476
• Main Authors: Chen, Hong, Fu, Zhi-Qiang, Chen, Lei, Qiu, Chun-Hui, Fu, Guang-Wei, Li, Ye, Shao, Dong-Hua, Feng, Xin-Gang, Lin, Jiao-Jiao
• Format: Journal Article
• Language: Chinese
• Published: China 01.12.2009
• Subjects: Animals; Female; Helminth Proteins - genetics; Helminth Proteins - immunology; Membrane Proteins - genetics; Membrane Proteins - immunology; Mice; Mice, Inbred BALB C; Plasmids; Recombinant Proteins - genetics; Recombinant Proteins - immunology; Schistosoma japonicum - genetics; Schistosoma japonicum - immunology; Schistosomiasis japonica - genetics; Schistosomiasis japonica - immunology
• ISSN: 1000-7423

To clone, express and characterize a tegument protein gene of Schistosoma japonicum (Sj29) , and investigate the immune protection of the recombinant protein against S. japonicum in mice. The gene coding for Sj29 protein was amplified by PCR, and the sequence was analyzed by bioinformatics tools. Partial fragment of Sj29 gene was subcloned into the prokaryotic expression vector pET28c(+). The recombinant plasmid was transformed into E. coli BL21 (DE3) and induced the recombinant with IPTG. The recombinant protein (rSj29) was purified by His-binding-resin affinity chromatography and characterized by Western blotting. Three groups each with 10 BALB/c mice were immunized subcutaneously three times (two weeks interval) respectively with 100 microl recombinant rSj29 (0.1 mg/ml) , adjuvant or PBS. At the 15th day after the final inoculation, each mouse was challenged by 40 +/- 2 cercariae of S. japonicum. At the 53rd day after infection, the mice were sacrificed to obtain the number of adult worms, number of eggs i