Effect of fibrinogen on the secretion of interleukin-1beta and - 8 by polymorphonuclear leukocytes

To investigate the effect of one of the acute-phase proteins, fibrinogen, on the release of IL-1beta and -8 by human peripheral polymorphonuclear leukocytes (PMN) and the possible role of fibrinogen during the destruction of periodontium. Peripheral PMN were isolated by discontinuous density gradien...

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Bibliographic Details
Published inZhonghua kouqiang yixue zazhi Vol. 43; no. 1; p. 8
Main Authors Ge, Song, Wu, Ya-fei, Liu, Tian-jia, Zhao, Lei, Meng, Shu
Format Journal Article
LanguageChinese
Published China 01.01.2008
Subjects
Cells, Cultured
Fibrinogen - pharmacology
Humans
Interleukin-1beta - metabolism
Interleukin-8 - metabolism
Middle Aged
Neutrophils - drug effects
Neutrophils - metabolism
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Summary:To investigate the effect of one of the acute-phase proteins, fibrinogen, on the release of IL-1beta and -8 by human peripheral polymorphonuclear leukocytes (PMN) and the possible role of fibrinogen during the destruction of periodontium. Peripheral PMN were isolated by discontinuous density gradient centrifuging technique. The freshly isolated PMN were suspended in Hank's balanced saline solution (1 x 10(9)/L) supplemented with 0.5% BSA and 0.1% glucose. The levels of IL-1beta and -8 in the supernatants produced by cultured cells upon the addition of human fibrinogen at different concentrations were measured by ELISA technique. Incubated with human fibrinogen at 2 g/L or 10 g/L for different time periods, human peripheral PMN released significantly greater amount of IL-1beta [(10.41 +/- 0.37) - (35.86 +/- 0.30) ng/L or (22.81 +/- 0.45) - (57.77 +/- 2.08) ng/L] and IL-8 [(93.90 +/- 13.95) - (2045.66 +/- 53.03) ng/L or (115.02 +/- 10.61) - (3858.69 +/- 25.65) ng/L] than PMN without the stimulation of fibrinoge
ISSN:1002-0098