Flow cytometric analysis of cerebrospinal fluid in the diagnosis of central nervous system leukemia

• Published in: Sichuan da xue xue bao. Journal of Sichuan University. Yi xue ban Vol. 41; no. 4; p. 664
• Main Authors: Jiang, Neng-Gang, Zhu, Huan-Ling, Zeng, Ting-Ting, Su, Jun, Zhang, Yao, Jia, Yong-Qian
• Format: Journal Article
• Language: Chinese
• Published: China 01.07.2010
• Subjects: Adolescent; Adult; Aged; Female; Flow Cytometry; Humans; Leukemia, Myeloid, Acute - cerebrospinal fluid; Male; Meningeal Neoplasms - cerebrospinal fluid; Meningeal Neoplasms - diagnosis; Meningeal Neoplasms - secondary; Middle Aged; Precursor Cell Lymphoblastic Leukemia-Lymphoma - cerebrospinal fluid; Precursor Cell Lymphoblastic Leukemia-Lymphoma - diagnosis; Young Adult
• ISSN: 1672-173X

To evaluate the value of flow cytometric immunophenotyping of cerebrospinal fluid (CSF) cells in the diagnosis of central nervous system leukemia. Ninety two CSF samples were analyzed with 4-color flow cytometry. Antibody panles CD19/CD34/CD3/CD45, CD117/CD34/CD5/CD45, CD7/CD34/ CD19/CD45, CD7/CD3/HLA-DR/CD45, CD20/CD10/CD3/CD45, and anti-g/anti-lambda/CD19/CD45 were used in determining cell composition and detecting abnormal cells. The results of flow cytometry were compared with conventional cell count and morphology. Flow cytometry analysis was repeated for five samples 48 hours after the initial test. Abnormal cells were found in 33 out of the 92 (35.9%) samples. Among the 59 samples taken from patients with lymphocyte neoplasm, CD19 + blast cells were found in the CSF in 13 patients with B-cell lymphoblastic leukemia; CD7+ blast cells were found in 4 T-ALL cases; and monoclonal CD19+ cells were found in 6 other types of lymphoma cases. In the 32 patients with clinically diagnosed myeloid leukemia, CD117+