Protein microarray analysis on changes in protein phosphorylation in recurrent nasopharyngeal carcinoma

• Published in: Zhong hua yi xue za zhi Vol. 91; no. 45; p. 3219
• Main Authors: Su, Hua-fang, Zhang, Xue-bang, Fang, Min, He, Qian-cheng, Li, Wen-feng, Wu, Shi-xiu
• Format: Journal Article
• Language: Chinese
• Published: China 06.12.2011
• Subjects: Adult; Carcinoma, Squamous Cell - metabolism; Carcinoma, Squamous Cell - pathology; Female; Gene Expression Profiling; Humans; Male; Middle Aged; Nasopharyngeal Neoplasms - metabolism; Nasopharyngeal Neoplasms - pathology; Neoplasm Recurrence, Local; Phosphorylation; Protein Array Analysis; Proteomics
• ISSN: 0376-2491
• DOI: 10.3760/cma.j.issn.0376-2491.2011.45.014

To compare the differential phosphorylation level of proteins between relapsed nasopharyngeal carcinoma (rNPC) and primary nasopharyngeal carcinoma (pNPC). Total protein was extracted from 4 pNPC tissue and 4 rNPC tissue samples from January 2003 to September 2005. Then it was analyzed by antibody microarray with 656 antibodies. The differential phosphorylation level of proteins was screened and clustering analysis conducted. The phosphorylation status of the protein sites and its functional pathways were analyzed via an online database of PhosphoSite Plus. The protein expressions were detected by immunohistochemistry. Relapsed and primary nasopharyngeal carcinomas had differential phosphorylation level of proteins. And 6 differentially expressed proteins were identified. The phosphorylation levels of KIT, ATP1A1, Synapsin, SEK1 and histone H2AX were up-regulated in rNPC (P = 0.007 - 0.048) while c-Jun was down-regulated (P = 0.030). The expression of P-H2AX in rNPC was significantly higher than that in pNPC