Neurotoxicity of beta-amyloid peptide 31-35 and 25-35 to cultured rat cortical neurons

To compare the toxicity of mechanism beta amyloid peptide (Abeta) 25-35 and 31-35 to cultured rat cortical neurons. The primary rat cerebral cortical neurons of rat were cultured 48 hours and randomly divided into control, Abeta25-35 (25 micromol/L)and Abeta31-35 (25 micromol/L) treated groups. Afte...

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Published inZhōnghuá yùfáng-yīxué zázhì Vol. 43; no. 12; p. 1081
Main Authors Zhang, Xiao-hong, Yu, Huan-ling, Xiao, Rong, Xiang, Li, Li, Li, Ma, Wei-wei, Zhang, Jie, Chu, Jin-hua
Format Journal Article
LanguageChinese
Published China 01.12.2009
Subjects
Amyloid beta-Peptides - toxicity
Animals
Apoptosis - drug effects
Cells, Cultured
Cerebral Cortex - cytology
Neurons - drug effects
Peptide Fragments - toxicity
Rats
Rats, Wistar
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Summary:To compare the toxicity of mechanism beta amyloid peptide (Abeta) 25-35 and 31-35 to cultured rat cortical neurons. The primary rat cerebral cortical neurons of rat were cultured 48 hours and randomly divided into control, Abeta25-35 (25 micromol/L)and Abeta31-35 (25 micromol/L) treated groups. After twenty four hours culturing, the cells were collected MTT assay was performed to measure the viability of cultured neurons. The mitochondrial membrane potential was determined to investigate the alteration of mitochondrial structure and function of neurons by laser scanning confocal microscope. The DNA damage of neurons was measured by single cell gel electrophoresis. The expressions of Bcl-2, Bax and p53 gene were measured by reverse transcriptase polymerase chain reaction (RT-PCR). Each experiment was repeated three times. The absorbance (0.746 +/- 0.071, 0.811 +/- 0.083) and fluorescence intensity (3.050 +/- 0.240, 2.806 +/- 0.203) of neurons in Abeta25-35 and 31-35 treated group were significantly lower (t(A)
ISSN:0253-9624