Application of saliva protein fingerprints in the diagnosis of oral squamous cell cancer by surface enhanced laser desorption ionization time of flight mass

• Published in: Zhonghua kouqiang yixue zazhi Vol. 44; no. 11; p. 664
• Main Authors: Sun, Gang, Ping, Fei-yun
• Format: Journal Article
• Language: Chinese
• Published: China 01.11.2009
• Subjects: Biomarkers, Tumor - chemistry; Carcinoma, Squamous Cell - diagnosis; Humans; Leukoplakia, Oral - diagnosis; Mass Spectrometry; Molecular Weight; Mouth Neoplasms - diagnosis; Peptide Mapping - methods; Proteomics; Saliva - chemistry; Sensitivity and Specificity; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
• ISSN: 1002-0098

To screen proteomic markers of oral precancerous lesion, local and metastatic squamous cell cancer from human saliva. Seventeen patients with oral squamous cell carcinoma, six cases with leukoplakia, seven cases with local metastasis and 15 healthy subjects were selected. All patients were diagnosed of T(1)N(0)M(0) and did not accept any preoperative treatment. Saliva samples were collected preoperatively, combined in the chips (CM-10) for two copies, and tested by surface enhanced laser desorption/ionization (SELDI) mass spectrometry respectively. Chips were detected by the PBS-II plus mass spectrometer reader. Mass accuracy was calibrated by the all-in-one peptide molecular mass standard (ciphergen ciosystems). The differentiated pattern between oral squamous cell carcinoma and healthy people consisted of four biomarker peaks of 5797, 2902, 3883, 4951 (mass/charge) with the sensitivity of 88.24% and specificity of 93.33%. The differentiated pattern between oral squamous cell carcinoma and leukoplakia consis