Interaction of vascular endothelial growth factor-C over-expression with tongue squamous cell carcinoma cell line Tca8113 with peri-carcinoma lymphatics

• Published in: Zhonghua kouqiang yixue zazhi Vol. 40; no. 2; p. 126
• Main Authors: Huang, Hong-zhang, Tang, Hai-kuo
• Format: Journal Article
• Language: Chinese
• Published: China 01.03.2005
• Subjects: Animals; Carcinoma, Squamous Cell - pathology; Cell Line, Tumor; Chick Embryo; Humans; Lymph Nodes - pathology; Lymphatic Metastasis; Lymphatic Vessels - drug effects; Lymphatic Vessels - pathology; Neck; Neoplasm Transplantation; Tongue Neoplasms - pathology; Transfection; Vascular Endothelial Growth Factor C - genetics
• ISSN: 1002-0098

To detect the role of Vascular endothelial growth factor C (VEGF-C) in the interaction of tongue squamous cell carcinoma (TSCC) with the peri-carcinoma lymphatics. TSCC cells were implanted on the chorioallantoic membrane (CAM), in which, group A was transfected by plasmid pREP7 including VEGF-C, group B was transfected by plasmid pREP7, and group C was Tca8113. Lymphatic vessels were stained by 5'-Nase. Morphologic analysis was used to evaluate the alteration of lymphatic vessels density (LVD), area and perimeter. The transplanted tumor well on CAM. The expression of VEGF-C in group A was higher than that in control group B and C. The LVD, perimeter and area in group A were (5.3 +/- 0.41)/high-power field, (148 +/- 21) microm and (76.8 +/- 13.5) microm(2) respectively in group A, which were significantly higher than that of group B and C (P < 0.01), while there were no significant difference between group B and C. CAM is ideal model for the research of lymphatic vessels; Over-expression of VEGF-C could induc