Whole cDNA sequence cloning and expression of chicken L-FABP gene and its relationship with lipid deposition of hybrid chickens

• Published in: Yíchuán Vol. 33; no. 7; p. 763
• Main Authors: Yu, Ying, Wang, Dong, Sun, Dong-Xiao, Xu, Gui-Yun, Li, Jun-Ying, Zhang, Yuan
• Format: Journal Article
• Language: Chinese
• Published: China 01.07.2011
• Subjects: Abdominal Fat - metabolism; Animals; Chickens - genetics; Chickens - growth & development; Chickens - metabolism; Cloning, Molecular; DNA, Complementary - genetics; Fatty Acid-Binding Proteins - genetics; Fatty Acid-Binding Proteins - metabolism; Female; Hybridization, Genetic; Liver - metabolism; Male; Molecular Sequence Data
• ISSN: 0253-9772
• DOI: 10.3724/SP.J.1005.2011.00763

Liver fatty acid-binding protein (L-FABP) is closely related to intracellular transportation and deposition of lipids. A positive differential displayed fragment was found in the liver tissue among Silkie (CC), CAU-brown chicken (CD), and their reciprocal hybrids (CD and DC) at 8 weeks-old using differential display RT-PCR techniques (DDRT-PCR). Through recycling, sequencing, and alignment analysis, the fragment was identified as chicken liver fatty acid-binding protein gene (L-FABP, GenBank accession number AY321365). Reverse Northern dot blot and semi-quantitative RT-PCR revealed that the avian L-FABP gene was over-expressed in the liver tissue of the reciprocal hybrids (CD and DC) compared to their parental lines (CC and DD), which was consistent with the fact that higher abdomen fat weight and wider inter-muscular fat width observed in the reciprocal hybrids. Considering the higher expression of L-FABP may contribute to the increased lipid deposition in the hybrid chickens, the functional study of avian L