Protective efficacy induced by dendritic cells pulsed with GST in combination with CpG oligodeoxynucleotide against Schistosoma japonicum infection

• Published in: Zhongguo jishengchongxue yu jishengchongping zazhi Vol. 28; no. 3; p. 185
• Main Authors: Li, Xiao-Hong, Cao, Jian-Ping, Tang, Lin-Hua, Xu, Yu-Xin, Liu, Shu-Xian, Wang, Sheng-Jun, Cheng, Jing
• Format: Journal Article
• Language: Chinese
• Published: China 30.06.2010
• Subjects: Animals; Antigens, Helminth - immunology; Dendritic Cells - immunology; Glutathione Transferase - immunology; Glutathione Transferase - therapeutic use; Helminth Proteins - immunology; Male; Mice; Mice, Inbred C57BL; Oligodeoxyribonucleotides - immunology; Oligodeoxyribonucleotides - therapeutic use; Schistosoma japonicum - immunology; Schistosomiasis japonica - immunology; Schistosomiasis japonica - prevention & control; Vaccines - immunology
• ISSN: 1000-7423

To study the protective effects on the infection of Schistosoma japonicum in C57BL/6 mice induced by dendritic cells DCs pulsed with GST in combination with CpG oligodeoxynucleotide. GST was purified and used to stimulate DC2.4 cell line. Antigen loading was analyzed by immunofluorescence method. Thirty-five C57BL/6 mice were divided into seven groups(5 mice per group). Mice in groups A, B, C, D and E were immunized subcutaneously with DCs, DCs treated with PSA, DCs pulsed with GST, DCs stimulated with GST+CpG ODN, DCs stimulated with CpG ODN, respectively. For the above five groups, each mouse received 100 microl cell suspension at the density of 10(7)/ml subcutaneously for three times at 2-week intervals. Each mouse of group F was immunized subcutaneously with 50 microg GST formulated in complete Freund's adjuvant first, and 50 microg, 10 microg GST respectively in incomplete Freund's adjuvant for the last two doses. Group G received PBS and served as control. Serum samples were collected 10 days after the