Expression of signal transducer and activator of transcription 6 in rat asthma model and the modulatory effect of dexamethasone

To study the expression of signal transducer and activator of transcription 6 and its mRNA in rat asthma model and the modulatory effect of dexamethasone (DXM). Thirty male SD rats were randomly divided into three groups: the control group, asthma group and DXM group. The rats in each group were sac...

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Published inZhonghua er ke za zhi Vol. 43; no. 7; p. 521
Main Authors Li, Chang-chong, Ye, Le-ping, Chen, Xiao-fang, Li, Shao-bo, Cai, Xiao-hong, Dong, Lin, Luo, Yun-chun, Zhang, Zheng-xia
Format Journal Article
LanguageChinese
Published China 01.07.2005
Subjects
Animals
Anti-Inflammatory Agents - pharmacology
Asthma - drug therapy
Asthma - genetics
Asthma - metabolism
Bronchi - cytology
Bronchoalveolar Lavage Fluid - cytology
Cell Count
Dexamethasone - pharmacology
Disease Models, Animal
Enzyme-Linked Immunosorbent Assay
Eosinophils - drug effects
Epithelial Cells - drug effects
Epithelial Cells - metabolism
Glucocorticoids - pharmacology
Immunohistochemistry
In Situ Hybridization
Interleukin-4 - metabolism
Male
Rats
Rats, Sprague-Dawley
RNA, Messenger
STAT6 Transcription Factor - genetics
STAT6 Transcription Factor - immunology
STAT6 Transcription Factor - metabolism
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Summary:To study the expression of signal transducer and activator of transcription 6 and its mRNA in rat asthma model and the modulatory effect of dexamethasone (DXM). Thirty male SD rats were randomly divided into three groups: the control group, asthma group and DXM group. The rats in each group were sacrificed 24 h after the last challenge. In the experiment, the rat model of asthma was established by ovalbumin (OVA) challenge method. The lung tissue was taken from the left lung, and bronchoalveolar lavage fluid (BALF) was collected from the right lung. The total cell numbers, eosinophils (EOS) count and differentiated cell counts in BALF were performed on different count fluids. The concentrations of IL-4 in serum and BALF were measured by using sandwich ELISA. The protein expressions of STAT6 were detected with immunohistochemical techniques. The mRNA expressions of STAT6 were detected with in situ hybridization. (1) The total cell counts in BALF, the absolute counts of EOS, and the ratios of eosinophils to the
ISSN:0578-1310