Changes in K+, Na+ and Cl- contents and K+ and Cl- fluxes during apoptosis of U937 cells by staurosporine. On the mechanism of cell dehydration in apoptosis

K+, Na+ and Cl- balance and K+ (Rb+) and 36Cl fluxes in during apoptosis of U937 cells caused by 0.2 or 1 microM staurosporine were studied by flame emission and radiotracer techniques. It is found that monovalent ion redistribution accounts for 2/3 of all decrease in the amount of intracellular osm...

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Published inT͡S︡itologii͡a Vol. 52; no. 7; p. 562
Main Authors Iurinskaia, V E, Goriachaia, T S, Rubashkin, A A, Shirokova, A V, Vereninov, A A
Format Journal Article
LanguageRussian
Published Russia (Federation) 2010
Subjects
Apoptosis
Chlorine - analysis
Chlorine - metabolism
Humans
Ion Transport
Ions - metabolism
Potassium - analysis
Potassium - metabolism
Protein Kinase Inhibitors - pharmacology
Rubidium - metabolism
Sodium - analysis
Sodium - metabolism
Staurosporine - pharmacology
U937 Cells
Water - metabolism
Water-Electrolyte Balance
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Summary:K+, Na+ and Cl- balance and K+ (Rb+) and 36Cl fluxes in during apoptosis of U937 cells caused by 0.2 or 1 microM staurosporine were studied by flame emission and radiotracer techniques. It is found that monovalent ion redistribution accounts for 2/3 of all decrease in the amount of intracellular osmolytes in apoptotic cells while 1/3 is due to the loss of other intracellular osmolytes. Na+ gain in apoptotic cells hampers dehydration is caused by K+ and Cl- loss. It is found that the rate of equilibration of 36Cl, Rb+ (K+) and 22Na+ between cells and the medium exceeds significantly the rate of alteration of cell ion content associated with apoptosis. It is concluded that apoptotic changes should be considered as a drift of the balanced ion distribution. Alteration of the ion balance in apoptosis, caused by 0.2 microM staurosporine, is associated with an increase in the uabain-resistant Rb+ (K+) "channel" influx and insignificant alteration of the uabain-sensitive "pump" influx. Stronger apoptosis, induced by 1 microM staurosporine, is associated with a decrease in the pump fluxes and insignificant changes in the "channel" Rb+ (K+) fluxes. Decreasing of the Cl- level in apoptotic cells by a factor 1.4-1.8 is accompanied with a decrease in the flux, by a factor 1.2-1.6.
ISSN:0041-3771