Study on the cellular and humoral immunity effect of recombinant Chinese hamster ovary cell hepatitis B vaccine in adults

• Published in: Zhōnghuá yùfáng-yīxué zázhì Vol. 44; no. 10; p. 918
• Main Authors: Zhang, Wei, Han, Li-li, Lin, Chang-ying, Li, Li-qiu, Gao, Pei, Lin, Hui, Gong, Xiao-hong, Huang, Fang, Tang, Ya-qing, Ma, Jian-xin, Zhang, Hai-yan, Wang, Chen, Yang, Peng, Li, Hui, Wu, Jiang, Sun, Mei-ping, He, Xiong, Pang, Xing-huo
• Format: Journal Article
• Language: Chinese
• Published: China 01.10.2010
• Subjects: Adolescent; Adult; Animals; Antibody Formation; CHO Cells; Cricetinae; Cricetulus; Double-Blind Method; Female; Hepatitis B - prevention & control; Hepatitis B Antibodies - blood; Hepatitis B Antibodies - immunology; Hepatitis B Vaccines - administration & dosage; Hepatitis B Vaccines - immunology; Humans; Immunity, Cellular - immunology; Male; Middle Aged; Young Adult
• ISSN: 0253-9624

To evaluate the cellular and humoral immunity effect of 10 µg and 20 µg recombinant Chinese hamster ovary (CHO) cell hepatitis B vaccine in adults by randomized double-blind controlled trials. A total of 642 adults aged 18 - 45 years old, non-vaccinated against hepatitis B, and hepatitis B five blood indicators negative were selected as the study subjects. The study subjects were randomly divided into two groups and each group had 321 subjects. The subjects were given 10 µg and 20 µg recombinant CHO hepatitis B vaccination respectively by 0, 1st, 6th month schedule. Blood sample was collected from each study subject one month after the second dose vaccination. The anti-HBs level was detected by Abbott chemiluminescence detection method (I2000) to evaluate humoral immunity status. Of all the study objects, 153 cases were randomly selected by the Excel random function. Their blood samples were collected and Lymphocyte were separated to detect the IL-4 and IFN-γ levels in vitro by enzyme-linked immunospot (ELISP