Salvianolic acid B alleviate the disruption of blood-brain barrier in rats after cerebral ischemia-reperfusion by inhibiting MAPK pathway

The aim of the study is to investigate the effect of salvianolic acid B (SalB) on blood-brain barrier (BBB) in rats after cerebral ischemia-reperfusion, and to illustrate its possible mechanisms. Cerebral ischemia-reperfusion was induced by middle cerebral artery occlusion in rats. The break-down of...

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Bibliographic Details
Published inYao hsüeh hsüeh pao Vol. 45; no. 12; p. 1485
Main Authors Li, Qin, Han, Li-pei, Li, Ze-hui, Zhang, Jun-tian, Tang, Min-ke
Format Journal Article
LanguageChinese
Published China 01.12.2010
Subjects
Animals
Benzofurans - isolation & purification
Benzofurans - pharmacology
Blood-Brain Barrier - drug effects
Blood-Brain Barrier - metabolism
Brain Ischemia - etiology
Brain Ischemia - metabolism
Brain Ischemia - pathology
Drugs, Chinese Herbal - isolation & purification
Drugs, Chinese Herbal - pharmacology
Infarction, Middle Cerebral Artery - complications
Male
MAP Kinase Kinase Kinase 1 - metabolism
MAP Kinase Signaling System - drug effects
Matrix Metalloproteinase 9 - metabolism
Nitric Oxide Synthase Type II - metabolism
p38 Mitogen-Activated Protein Kinases - metabolism
Phosphorylation
Plants, Medicinal - chemistry
Random Allocation
Rats
Rats, Sprague-Dawley
Reperfusion Injury - metabolism
Reperfusion Injury - pathology
Salvia miltiorrhiza - chemistry
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Summary:The aim of the study is to investigate the effect of salvianolic acid B (SalB) on blood-brain barrier (BBB) in rats after cerebral ischemia-reperfusion, and to illustrate its possible mechanisms. Cerebral ischemia-reperfusion was induced by middle cerebral artery occlusion in rats. The break-down of BBB was indicated by extravasations of immunoglobulin (IgG) monitored with immunohistochemistry. The expression of MMP-9 and NOS2 in the brain was determined by immunohistochemistry, and the expression of p-p38 and p-ERK1/2 was detected by Western blotting. It was shown that on day 2 after ischemia-reperfusion the IgG accumulated around the vascular boundary zone, suggesting the break-down of BBB, and the expression of MMP-9 and NOS2 up-regulated at the same time. The result of Western blotting suggested that the expression of p-p38 and p-ERK1/2 increased. On day 7 after ischemia-reperfusion the. expression of MMP-9 and NOS2 was about the same level as day 2, the expression of p-p38 was higher than that on day 2 a
ISSN:0513-4870