Fosinopril up-regulates and ameliorates the Ang II induced down-expression of klotho gene in NRK-52E

• Published in: Zhongguo ying yong sheng li xue za zhi Vol. 26; no. 3; p. 348
• Main Authors: Lin, Shu-Dian, Zhou, Qiao-Ling, Zhan, Feng, Chen, Dao-Jun, Li, Wen-Ning
• Format: Journal Article
• Language: Chinese
• Published: China 01.08.2010
• Subjects: Angiotensin II - pharmacology; Animals; Cells, Cultured; Down-Regulation; Epithelial Cells - drug effects; Epithelial Cells - metabolism; Fosinopril - pharmacology; Gene Expression - drug effects; Glucuronidase - genetics; Glucuronidase - metabolism; Kidney Tubules - cytology; Kidney Tubules - drug effects; Kidney Tubules - metabolism; Rats; RNA, Messenger - genetics
• ISSN: 1000-6834

To investigate the effect of fosinopril (Fos) on regulating klotho gene expression and elucidate the mechanism of Fos regulating the Angiotensin II (AngII) -induced down-expression of klotho gene. Culture cells, NRK-52E, were incubated with media either AngII or Fos or both of all. Experimental groups incubated with Fos (10(-5) mol/L) were divided according to variant points of time for 0 (control), 3, 6, 12, 24 h. Different concentration of Fos was selected to incubated with culture cells for 0 (control), 10(-9) 10(-8), 10(-7), 10(-6), 10(-5) mol/L at the optimal time point (24 h). Five groups, which were A: control; B: AngII (10(-7) mol/L); C: Fos(10(-5) mol/L); D: AngII (10(-7) mol/L) + Fos(10(-5) mol/L) and E: Cells pretreated with Fos(10(-5) mol/L)12 h incubated with AngII (10(-7) mol/L) were divided to observe the effect of Fos on expression of klotho induced by AngII. RT-PCR and immunohistochemistry (IHC) were applied to evaluate the klotho mRNA and protein expression, respectively. Fos up-regulated kl